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Dipartimento di Fisica

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Correlative microscopy techniques to study light-induced damage on amyloid fibers
Speaker Speaker: Caterina Maria Tone
Affiliato Affiliato: Instituto Madrileņo de Estudios Avanzados en Nanociencia (IMDEA Nano), Madrid, Spain
Data Evento Giovedì 16 Maggio , alle ore 15.30 - Aula Seminari, Cubo 31C
In recent years amyloid fibrils have been progressively gaining an important role in nanotechnology and biomaterial applications as a consequence of their unique physical and mechanical properties. However, these β-sheet rich protein aggregates are also strongly associated with various neurodegenerative diseases, such as Alzheimer's and Parkinson's or amyloidosis. In this scenario, the possibility to characterize these structures at the nanoscale is crucial to gain a more complete picture on biological systems. In this talk I will show our recent studies on the possibility to use photoactive compounds that selectively target the β structures, without affecting the fraction of functional protein, as an alternative to degrade them. A compound based on Thioflavin T (ROS-ThT), which selectively targets pathogenic aggregates also in the presence of other proteins, has been used during the experiments. Under visible irradiation, this compound is activated producing both reactive oxygen species (ROS) and fluorescence emission.
A combined study with Atomic Force Microscopy and correlative fluorescence techniques has been used to analyze the photodynamic damage induced by ROS-ThT on amyloid fibers, obtaining information, at the nanoscale, on the light-induced breakage of the amyloid fibers. The results obtained from these studies could improve phototherapeutic strategies for amyloid-related disorders.